Glycolysis and respiration in yeasts
نویسندگان
چکیده
1. Simultaneous and continuous measurements of changes in CO2 and 02 concentrations in glucose-metabolizing yeast suspensions by mass spectrometry enabled a study of the Pasteur effect (aerobic inhibition of glycolysis) in Saccharomyces uvarum and Schizosaccharomyces pombe. 2. A different control mechanism operates in Candida utilis to give a damped oscillation after the anaerobic-aerobic transition. 3. The apparent Km values for respiration of the three yeasts were in the range 1.3-1.8 pM-02. 4. The apparent Km values for 02 of the Pasteur effect were 5 and 13 pM for catabolite-repressed and derepressed S. uvarum respectively and 7 UM for Sch. pombe. 5. These results are discussed with respect to currently accepted mechanisms for the control of glycolysis.
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